Interference in PCR: Transcription amplifications of mixed PVY isolates

Primer design versus PCR bias in methylation independent PCR amplifications.

Many protocols in methylation studies utilize one primer set to generate a PCR product from bisulfite modified template regardless of its methylation status (methylation independent amplification MIP). However, proportional amplification of methylated and unmethylated alleles is hard to achieve due to PCR bias favoring amplification of unmethylated relatively GC poor sequence. Two primer design...

Heteroduplexes in mixed-template amplifications: formation, consequence and elimination by 'reconditioning PCR'.

Although it has been recognized that PCR amplification of mixed templates may generate sequence artifacts, the mechanisms of their formation, frequency and potential elimination have not been fully elucidated. Here evidence is presented for heteroduplexes as a major source of artifacts in mixed-template PCR. Nearly equal proportions of homoduplexes and heteroduplexes were observed after co-ampl...

on the status of mixed methods research in applied linguistics

چکیده ندارد.

One-Step Reverse Transcription PCR (RT-PCR). Fatemeh Mehrpooyan*

Biological, serological and molecular typing of potato virus Y (PVY) isolates from Tunisia.

In Tunisia, potato virus Y (PVY) currently presents a significant threat to potato production, reducing tuber yield and quality. Three hundred and eighty-five potato samples (six different cultivars) collected in autumn 2007 from nine regions in Tunisia were tested for PVY infection by DAS-ELISA. The virus was detected in all regions surveyed, with an average incidence of 80.26%. Subsequently, ...